Peer-reviewed and review articles
Deshaies Lab |
2018
160. Verma R., Reichermeier K.M., Burroughs A.M., Oania R.S., Reitsma J.M., Aravind L., Deshaies R.J. (2018). Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes. Nature. 557, 446-51.
159. Reiterer V., Figueras-Puig C., Le Guerroue F., Confalonieri S., Vecchi M., Jalapothu D., Kanse S.M., Deshaies R.J., Di Fiore P.P., Behrends C., Farhan H. (2018). The pseudophosphatase STYX targets the F-box of FBXW7 and inhibits SCFFBXW7function. EMBO J. 15;37(6).
158. Liu X., Reitsma J.M., Mamrosh J.L., Zhang Y., Straube R., Deshaies R.J. (2018). Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 69, 773-786.
2017
157. Skrott Z, Mistrik M, Andersen KK, Friis S, Majera D, Gursky J, Ozdian T, Bartkova J, Turi Z, Moudry P, Kraus M, Michalova M, Vaclavkova J, Dzubak P, Vrobel I, Pouckova P, Sedlacek J, Miklovicova A, Kutt A, Li J, Mattova J, Driessen C, Dou QP, Olsen J, Hajduch M, Cvek B, Deshaies RJ, Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature 552, 194-199.
156. Nakasone M.A., Lewis T.A., Walker O., Thakur A., Mansour W., Castañeda C.A., Goeckeler-Fried J.L., Parlati F., Chou T.F., Hayat O., Zhang D., Camara C.M., Bonn S.M., Nowicka U.K., Krueger S., Glickman M.H., Brodsky J.L., Deshaies R.J., Fushman D. (2017). Structural Basis for the Inhibitory Effects of Ubistatins in the Ubiquitin-Proteasome Pathway. Structure 25, 1839-1855.
155. Reitsma J.M., Liu X., Reichermeier K.M., Moradian A., Sweredoski M.J., Hess S., Deshaies R.J. (2017). Composition and Regulation of the Cellular Repertoire of SCF Ubiquitin Ligases. Cell. 171, 1326-1339.
154. Blythe, E.E., Olson, K.C., Chau, V. and Deshaies, R.J. (2017). Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP-NPLOC4-UFD1L is enhanced by a mutation that causes multisystem proteinpathy. Proc. Natl. Acad. Sci. U S A. E-published ahead of print May 16, 2017.
153. Lauinger, L., Li, J., Shostak, A., Cemel, I.A., Ha, N., Zhang, Y., Merkl, P.E., Obermeyer, S., Stankovic-Valentin, N., Schafmeier, T., Wever, W.J., Bowers, A.A., Carter, K.P., Palmer, A.E., Tschochner, H., Melchior, F., Deshaies, R.J., Brunner, M., and Diernfellner, A. (2017). Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat. Chem. Biol. E-published ahead of print May 1, 2017.
152. Nguyen, T.V., Li, J., Lu, C.J., Mamrosh, J.L., Lu, G., Cathers, B.E., and Deshaies, R.J. (2017). P97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc. Natl. Acad. Sci. U S A 14, 3565-3571.
151. Li, J., Yakushi, T., Parlati, F., Mackinnon, A.L., Perez, C., Ma, Y., Carter, K.P., Colayco, S., Magnuson, G., Brown, B., Nguyen, K., Vasile, S., Suyama, E., Smith, L.H., Sergienko, E., Pinkerton, A.B., Chung, T.D., Palmer, A.E., Pass, I., Hess, S., Cohen, S.M., and Deshaies RJ. (2017). Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11. Nat. Chem. Biol. 60, 1343-1361. Journal
150. Perez, C., Li, J., Parlati, F., Rouffet, M., Ma, Y., Mackinnon, A.L., Chou, T.F., Deshaies, R.J. and Cohen, S.M. (2017). Discovery of an Inhibitor of the Proteasome Subunit Rpn11. J. Med. Chem. 4, 1343-1361. Correction: J. Med. Chem. 7, 3217. Journal
2016
149. Reiterer, V, Figueras-Puig C., Le Guerroue F., Confalonieri, S., Vecchi, M., Jalapothu, D., Kanse, S.M., Deshaies, R.J., Di Fiore, P.P., Behrends, C. and Farhan, H. (2016). The pseudophosphatase STYX targets the F-box of FBXW7 and inhibits SCFFBXW7 function. EMBO J. 36, 260-273. Journal
148. Vangala, J.R, Sotzny, F., Kruger, E., Deshaies, R.J. and Radhakrishnan, S.K. (2016). Nrf1 can be processed and activated in a proteasome-independent manner. Curr. Biol. 26, R834-835. Journal
147. Kleiger, G. and Deshaies, R. (2016). Tag Team Ubiquitin Ligases. Cell 166, 1080-1081. Journal
146. Sung, M.K., Porras-Yakushi, T.R., Reitsma, J.M., Huber, F.M., Sweredoski, M.J., Hoelz, A., Hess, S. and Deshaies, R.J. (2016). A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. eLife 5, eLife.19105. PMC / Journal
145. Xue, L., Blythe, E.E., Freiberger, E.C., Mamrosh, J., Hebert, A.S., Reitsma, J.M., Hess, S., Coon, J.J. and Deshaies, R.J. VCP-adaptor interactions are exceptionally dynamic and subject to differential modulation by a VCP inhibitor. Mol. Cell. Proteomics 15 2970-2986. Journal
144. Sung, M.K., Reitsma, J.M., Sweredoski, M.J., Hess, S. and Deshaies, R.J. (2016). Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome system. Mol. Biol. 27, 2642-2652. PMC / Journal
143. Mosadeghi, R., Reichermeier, K.M., Winkler, M., Schreiber, A., Reitsma, J.M., Zhang, Y., Stengel, F., Cao, J., Kim, M., Sweredoski, M.J., Hess, S., Leitner, A., Aebersold, R., Peter, M., Deshaies, R.J. and Enchev, R.I. (2016). Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. eLife 5, e12102. PMC / Journal
142. Nguyen, T.V., Lee, J.E., Sweredoski, M.J., Yang, S.J., Jeon, S.J., Harrison, J.S., Yim, J.H., Lee, S.G., Handa, H., Kuhlman, B., Jeong, J.S., Reitsma, J.M., Park, C.S., Hess, S. and Deshaies, R.J. (2016). Glutamine triggers acetylation-dependent degradation of glutamine synthetase via the thalidomide receptor cereblon. Mol. Cell 6, 809-820. Journal
141. Banerjee, S., Bartesaghi, A., Merk, A., Prashant, R., Bulfer, S. L., Yan, Y., Green, N., Mroczkowski, B., Neitz, R.J., Wipf, P., Falconieri, V., Deshaies, R.J., Milne, J.L.S., Huryn, D., Arkin, M. and Subramaniam, S. (2016). 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science 351, 871-875. Journal
2015
140. Alverez, C., Bulfer, S.L., Chakrasali, R., Chimenti, M.S., Deshaies R.J., Green, N., Kelly, M., LaPorte, M.G., Lewis, T.S., Liang, M., Moore, W.J., Neitz, R.J., Peshkov, V.A., Walters, M.A., Zhang, F., Arkin, M.R., Wipf, P. and Huryn, D.M. (2016). Allosteric indoleamide inhibitors of p97: Identification of a novel probe of the ubiquitin pathway. ACS Med. Chem. Lett. 2, 182-187. PMC / Journal
139. Deshaies, R.J. (2015). Protein degradation: Prime time for PROTACs. Nat. Chem. Biol. 11, 634-635. Journal
138. Pulvino, M., Chen, L., Oleksyn, D., Li, J., Compitello, G., Rossi, R., Spence, S., Balakrishnan, V., Jordan, C., Poligone, B., Casulo, C., Burack, R., Shapiro, J.L., Bernstein, S., Friedberg, J.W., Deshaies, R.J., Land, H., and Zhao, J. Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. (2015). Oncotarget 6, 14796-14813. PMC / Journal
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2014
137. Deshaies, R.J. (2014). Proteotoxic crisis, the ubiquitin-proteasone system, and cancer therapy. BMC Biol. 12:94. PMC / Journal
136. Fang, N.N., Chan, G.T., Zhu, M., Comyn, S.A., Persaud, A., Deshaies, R.J., Rotin, D., Gsponer, J., Mayor, T. (2014).
Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat. Cell Biol. 16, 1227-1237. PMC / Journal
135. Deshaies, RJ. Structural biology: Corralling a protein-degradation regulator. (2014). Nature 512, 145-146. Journal
134. Chan, N.C., den Besten, W., Sweredoski, M.J., Hess, S., Deshaies, R.J., and Chan, D.C. (2014). Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2. J. Biol. Chem. 289, 19789-19798. PMC / Journal
133. Chou, T.F., Bulfer, S.L., Weihl, C.C., Li, K., Lis, L.G., Walters, M.A., Schoenen, F.J., Lin, H.J., Deshaies, R.J., and Arkin, M.R. (2014). Specific inhibition of p97/VCP ATPase and kinetic analysis demonstrate interaction between D1 and D2 ATPase domains. J. Mol. Biol. 426, 2886-2899. PMC / Journal
132. Honarpour, N., Rose, C.M., Brumbaugh, J., Anderson, J., Graham, R.L., Sweredoski, M.J., Hess, S., Coon, J.J., and Deshaies, R.J. (2014). FBXL16 binds PP2A and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol. Cell. Proteomics 13, 780-791. PMC / Journal
131. Radhakrishnan, S.K., den Besten, W. and Deshaies, R.J. (2014). p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition. eLife 3, e01856. PMC / Journal
2013
130. Kolawa, N.J., Sweredoski, M.J., Graham, R.L.J., Oania, R., Hess, S., and Deshaies, R.J. (2013). Perturbations to the ubiquitin conjugate proteome in yeast Dubx mutants identify Ubx2 as a regulator of membrane lipid composition. Mol. Cell. Proteomics 12, 2791-2803. PMC / Journal
129. Kurimchak, A., Haines, D.S., Garriga, J., Wu, S., De Luca, F., Sweredoski, M.J., Deshaies, R.J., Hess, S., and Graña, A. (2013). Activation of p107 by FGF, which is essential for chondrocyte cell cycle exit, is mediated by the PP2A/B55a holoenzyme. Mol. Cell. Biol. 33, 3330-3342. PMC / Journal
128. Chou, T.F., Li, K., Nordin, B.E., Porubsky, P., Frankowski, K., Patricelli, M.P., Aubé, J., Schoenen, F.J., and Deshaies, R. (2013). Selective, reversible inhibitors of the AAA ATPase p97. Probe Reports from the NIH Molecular Libraries Program [Internet], National Center for Biotechnology Information, 2010-April 14, 2011 (updated May 1, 2013). Report
127. Pierce, N.W., Lee, J.E., Liu, X., Sweredoski, M.J., Graham, R.L., Larimore, E.A., Rome, M., Zheng, N., Clurman, B.E., Hess, S., Shan, S.O., and Deshaies, R.J. (2013). Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins. Cell 153, 206-215. PMC / Journal
126. Verma, R., Oania, R.S., Kolawa, N.J., and Deshaies, R.J. (2013). Cdc48/p97 promotes degradation of aberrant nascent polypeptides bound to the ribosome. eLife 2, e00308. PMC / Journal
125. Chou, T.F., Li, K., Frankowski, K.J., Schoenen, F.J., and Deshaies, R.J. (2013). Structure-activity relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase. Chem. Med. Chem. 8, 297-312. PMC / Journal
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2012
124. Oh, Y., Chang, K.J., Orlean, P., Wloka, C., Deshaies, R., and Bi, E. (2012). Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol. Biol. Cell. 23, 2445-2456. PMC / Journal
123. Emberley, E.D., Mosadeghi, R., and Deshaies, R.J. (2012). Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-Fbox and substrate, and CSN inhibits deneddylated SCF by a non-catalytic mechanism. J. Biol. Chem. 287, 29679-29689. PDF / Journal
122. Besten, W.D., Verma, R., Kleiger, G., Oania, R.S., and Deshaies, R.J. (2012). NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat. Struct. Mol. Biol. 19, 511-516. PMC / Journal
121. Sohn, C.H., Agnew, H.D., Lee, J.E., Sweredoski, M.J., Graham, R.L., Smith, G.T., Hess, S., Czerwieniec, G., Loo, J.A., Heath, J.R., Deshaies, R.J., and Beauchamp, J.L. (2012). Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions. Anal. Chem. 84, 2662-2669. PMC / Journal
120. Haines, D.S., Lee, J.E., Beauparlant, S.L., Kyle, D.B., den Besten, W., Sweredoski, M.J., Graham, R.L., Hess, S., and Deshaies, R.J. (2012). Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking. Mol. Cell. Proteomics 11, M111.016444. PMC / Journal
119. Sohn, C.H., Lee, J.E., Sweredoski, M.J., Graham, R.L., Smith, G.T., Hess, S., Czerwieniec, G., Loo, J.A., Deshaies, R.J., Beauchamp, J.L. (2012). Click chemistry facilitates formation of reporter ions and simplified synthesis of amine-reactive multiplexed isobaric tags for protein quantification. J. Am. Chem. Soc. 134, 2672-2680. PMC / Journal
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2011
118. Rabut, G., Le Dez, G., Verma, R., Makhneyvych, T., Knebel, A., Kurz, T., Boone, C., Deshaies, R.J. and Peter, M. (2011) The TFIIH subunit Tfb3 regulates cullin neddylation. Mol. Cell. 43, 488-495. PMC / Journal
117. Gomez, T.A., Kolawa, N., Gee, M., Sweredoski, M.J. and Deshaies, R.J. (2011). Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1. BMC Biol. 9, 33. PMC / Journal
116. Chou, T.F. and Deshaies, R.J. (2011). Development of p97 AAA ATPase inhibitors. Autophagy 7, 1091-1092. PMC / Journal
115. Chou, T.F. and Deshaies, R.J. (2011). Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system. J. Biol. Chem. 286, 16546-16554. PMC / Journal
114. Lee, J.E., Sweredoski, M.J., Graham, R.L., Kolawa, N.J., Smith, G.T., Hess, S., and Deshaies, R.J. (2011). The steady-state repertoire of human SCF Ubiquitin ligase complexes does not require ongoing Nedd8 conjugation. Mol. Cell. Proteomics 10, 6460, 1-9. PMC / Journal
113. Saha, A., Lewis, S., Kleiger, G., Kuhlman, B., and Deshaies, R.J. (2011). Essential role for ubiquitin-ubiquitin-conjugating enzyme interaction in ubiquitin discharge from Cdc34 to substrate. Mol. Cell 42, 75-83. PMC / Journal
112. Chou, T.F., Brown, S.J., Minond, D., Nordin, B.E., Li, K., Jones, A.C., Chase, P., Porubsky, P.R., Stoltz, B.M., Schoenen, F.J., Patricelli, M.P., Hodder, P., Rosen, H., and Deshaies, R.J. (2011) Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc. Natl. Acad. Sci. USA 108, 4834-4839. PMC / Journal
111. Verma, R., Oania, R., Fang, R., Smith, G.T., and Deshaies, R.J. (2011). Cdc48/p97 mediates UV-dependent turnover of RNA Pol II. Mol. Cell 41, 82-92. PMC / Journal
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2010
110. Deshaies, R.J., Emberley, E.D., and Saha, A. (2010). Control of cullin-ring ubiquitin ligase activity by Nedd8. Subcell. Biochem. 54, 41-56. Journal
109. Tyrell, A., Flick, K., Kleiger, G., Zhang, H., Deshaies, R.J. and Kaiser, P. (2010). Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins. Proc. Natl. Acad. Sci. USA 107, 19796-19801. PMC / Journal
108. Collins, G.A., Gomez, T.A., Deshaies, R.J., and Tansey, W.P. (2010). Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast 27, 965-974. PMC / Journal
107. Aghajan, M., Jonai, N., Flick, K., Fu, F., Luo, M., Cai, X., Ouni, I., Pierce, N., Tang, X., Lomenick, B., Damoiseaux, R., Hao, R., Del Moral, P.M., Verma, R., Li, Y., Li, C., Houk, K.N., Jung, M.E., Zheng, N., Huang, L., Deshaies, R.J., Kaiser, P., and Huang, J. (2010). Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat. Biotechnol. 28, 738-742. PMC / Journal
106. Radhakrishnan, S.K., Lee, C.S., Young, P., Beskow, A, Chan, J.Y., and Deshaies, R.J. (2010). Transcription factor Nrf1 mediates the proteasome recovery pathway after proteasome inhibition in mammalian cells. Mol. Cell 38, 17-28. PMC / Journal
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2009
105. Pierce, N., Kleiger, G., Shan, S., and Deshaies, R.J. (2009). Detection of sequential ubiquitination on a millisecond time-scale. Nature 462, 615-619. PMC / Journal
104. Kleiger, G., Saha, A., Lewis, S., Kuhlman, B., and Deshaies, R.J. (2009). Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates. Cell 139, 957-968. PMC / Journal
103. Kleiger, G., Hao, B., Mohl, D.A., and Deshaies, R.J. (2009). The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCDC4. J. Biol. Chem. 284, 36012-36023. PMC / Journal
102. Deshaies, R.J. and Joazeiro, C.A.P. (2009). RING domain E3 ubiquitin ligases. Annu. Rev. Biochem. 78, 399-434. PDF
101. Deshaies, R.J. (2009). Drug discovery: Fresh target for cancer therapy (News & Views). Nature 458, 709-710. PDF
100. Oh, Y.M., Kwon, Y.E., Kim, J.M., Bae, S.J., Lee, B.K., Yoo, S.J., Chung, C.H., Deshaies R.J., Seol, J.H. (2009) Chfr is linked to tumour metastasis through the downregulation of HDAC1. Nat. Cell Biol. 11, 295-302. PDF
99. Machida, K., Tsukamoto, H., Mkrtchyan, H., Duan, L., Dynnyk, A., Liu, H.M., Asahina, K., Govindarajan, S., Ray, R., Ou, J.H., Seki, E., Deshaies, R., Miyake, K., and Lai, M.M. (2009). Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog. Proc. Nat. Acad. Sci. USA 106, 1548-1553. PMC / Journal
98. Mohl, D.A., Huddleston, M.J., Collingwood, T.S., Annan, R.S., and Deshaies, R.J. (2009). Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. J. Cell Biol. 184, 527-539. PMC / Journal
97. Collins, G.A., Lipford, J.R., Deshaies, R.J. and Tansey, W.P. (2009). Gal4 turnover and transcription activation (Brief Communication Arising). Nature 461, E7. PMC / Journal
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2008
96. Rodriguez-Gonzalez, A., Cyrus, K., Salcius, M., Kim, K., Crews, C.M., Deshaies, R.J., and Sakamoto, K.M. (2008). Targeting steroid hormone receptors for ubiquitination and degradation in breast and prostate cancer. Oncogene 27, 7201-7211. Journal
954. Saha, A. and Deshaies, R. J. (2008). Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. Mol. Cell 32, 21-31. PMC / Journal
94. Alexandru, G., Graumann, J., Smith, G.T., Kolawa, N.J., Fang, R., and Deshaies, R.J. (2008). UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1a turnover. Cell 134, 804-816. PMC / Journal
93. Goh, A.M., Walters, J.K., Elsasser, S., Verma, R., Deshaies, R.J., Finley, D., and Howley, P.M. (2008). Components of the ubiquitin-proteasome pathway compete for surfaces on Rad32 family proteins. BMC Biochem. 9, 4. PMC / Journal
92. Haririnia, A., Verma, R., Purohit, N., Twarog, M.Z., Deshaies, R.J., Bolon, D. and Fushman, D. (2008). Mutations in the hydrophobic core of ubiquitin differentially affect its recognition by receptor proteins. J. Mol. Biol. 375, 979-996. PMC / Journal
2007
91. Mayor, T., Graumann, J., Bryan, J., Maccoss, M.J., and Deshaies, R.J. (2007). Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway. Mol. Cell. Proteomics 6, 1885-1895. Journal
90. Ghaboosi, N. and Deshaies, R.J. (2007). A conditional yeast E1 mutant blocks the ubiquitin-proteasome pathway and reveals a role for ubiquitin conjugates in targeting Rad23 to the proteasome. Mol. Biol. Cell 18, 1953-63. PMC / Journal
2006
89. Petroski, M.D., Kleiger, G., and Deshaies, R.J. (2006). Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex. Mol. Cell 24, 523-534. Journal
88. Sharon, M., Taverner, T., Ambroggio, X.I., Deshaies, R.J., and Robinson, C.V. (2006). Structural organization of the 19% proteasome lid: Insights from MS of intact complexes. PloS Biol. 4, 1314-1323 (e267). PMC / Journal
87. Cope, G.A. and Deshaies, R.J. (2006). Targeted silencing of JabI /Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels. BMC Biochem. 7, 1. PMC / Journal
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2005
86. Petroski, M.D. and Deshaies, R.J. (2005). Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34. Cell 123, 1107-1120. Journal
85. Petroski, M.D. and Deshaies, R.J. (2005). In vitro reconstitution of SCF substrate ubiquitination with purified proteins. Method. Enzymol. 398, 143-158. PDF
84. Verma, R. and Deshaies, R.J. (2005). Assaying degradation and deubiquitination of a ubiquitinated substrate by purified 26S proteasomes. Method. Enzymol. 398, 391-399. PDF
83. Mayor, T. and Deshaies, R.J. (2005). Two-step affinity purification of multiubiquitylated proteins from Saccharomyces cerevisiae. Method. Enzymol. 399, 385-392. PDF
82. Mah, A.S., Elia, A.E.H., Devgan, G, Ptacek, J., Schutkowski, M., Snyder, M., Yaffe, M.B., and Deshaies, R.J. (2005). Substrate specificity analysis of protein kinase complex Dbf20Mob1 by peptide library and proteome array screening. BMC Biochem. 6, 22. PMC / Journal
81. Lipford, J.R., Smith, G.T., Chi, Y., and Deshaies, R.J. (2005). A putative stimulatory role for activator turnover in gene expression. Nature 438, 113-116. PDF
80. Mayor, T., Lipford, J.R., Graumann, J., Smith G.T., and Deshaies, R.J. (2005). Analysis of poly-ubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets. Mol. Cell. Proteomics 4, 741-751. Journal
79. Petroski, M.D. and Deshaies, R. J. (2005). Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell. Biol. 6, 9-20. PDF
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2004
78. Verma, R., Peters, N.R., Tochtrop, G.P., Sakamoto, K.M., D’Onofrio, M., Varadan, R., Fushman, D., Deshaies, R.J., and King, R.W. (2004). Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain. Science 306, 117-120. Journal
77. Azzam, R., Chen, S.L., Shou, W., Mah, A.S., Alexandru, G., Nasmyth, K., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2004). Phosphorylation by cyclin B-Cdk underlies release of mitotic exit. Science 305, 516-519. Journal
76. Verma, R., Oania, R., Graumann, J., and Deshaies, R.J. (2004). Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system. Cell 118, 99-110. Journal
75. Wertz, I.E., O'Rourke, K.M., Zhang, Z., Dornan, D., Arnott, D., Deshaies, R.J., and Dixit, V.M. (2004). Human De-etiolated-1 regulates cJun by assembling a CUL 4A ubiquitin ligase. Science 303, 1371-1374. Journal
74. Schneekloth, J.S. Jr., Fonseca, F.N., Koldobskiy, M., Mandal, A., Deshaies, R., Sakamoto, K. and Crews, C.M. (2004). Chemical genetic control of protein levels: selective in vivo targeted degradation. J. Am. Chem Soc. 126, 3748-3754. PDF
73. Graumann, J., Dunipace, L.A., Seol, J.H., McDonald, W.H., Yates, J.R. III, Wold, B.J., and Deshaies, R.J. (2004). Applicability of TAP-MudPIT to pathway proteomics in yeast. Mol. Cell. Proteomics 3, 266-237. Journal
72. Baumeister, W., Bachmair, A., Chau, V., Cohen, R., Coffino, P., DeMartino, G., Deshaies, R., et al. (2004). Varshavsky’s Contributions (in Letters). Science 19, 1290-1292. Journal
71. Ambroggio, X.I., Rees, D.C., and Deshaies, R.J. (2004). JAMM: A metalloprotease-like zinc site in the proteasome and signalosome. PLoS Biol. 2, 0113-0119. PMC / Journal
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2003
70. Sakamoto, K.M., Kim, K.B., Verma, R., Ransick, A., Stein, B., Crews, C.M., and Deshaies, R.J. (2003). Development of protacs to target cancer-promoting proteins for ubiquitination and degradation. Mol. Cell. Proteomics 2, 1350-1358. Journal
69. Lipford, J.R., and Deshaies, R.J. (2003). Diverse roles for ubiquitin-dependent proteolysis in transcriptional activation. Nat. Cell Biol. 5, 845-50. PDF
68. Cope, G.A., and Deshaies, R.J. (2003). COP9 signalosome: a multifunctional regulator of SCF and other cullin-based ubiquitin ligases. Cell 114, 663-71. Journal
67. Petroski, M.D, and Deshaies, R.J. (2003). Redundant degrons ensure the rapid destruction of sic1 at the g1 /s transition of the budding yeast cell cycle. Cell Cycle 2, 410-1. Journal
66. Petroski, M.D, and Deshaies, R.J. (2003). Context of multiubiquitin chain attachment influences the rate of Sic1 degradation. Mol. Cell 11, 1435-44. Journal
65. Park C.J., Song, S., Lee P.R., Shou W., Deshaies R.J. and Lee K.S (2003). Loss of CDC5 function in saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics 163, 21-33. PMC / Journal
2002
64. Cope, G.A., Suh, G.S., Aravind, L., Schwarz, S.E., Zipursky, S.L., Koonin, E.V. and Deshaies, R.J. (2002). Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of NEDD8 from CUL1. Science 298, 608-611. Journal
63. Verma, R., Aravind L., Oania, R., McDonald, W.H., Yates, J. R. III, Koonin, E.V. and Deshaies, R.J. (2002). Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298, 611-615. Journal
62. Loughrey Chen S., Huddleston, M.J., Shou, W., Deshaies, R.J., Annan, R.S., and Carr, S.A. (2002). Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Mol. Cell. Prot. 1, 186-96. Journal
61. Shou, W., Verma, R., Annan, R.S., Huddleston, M.J., Chen, S.L., and Deshaies R.J. (2002). Mapping phosphorylation sites in proteins by mass spectrometry. Method. Enzymol. 351, 279-296. Preprint
60. Shou, W., Azzam, R., Chen, S.L., Huddleston, M.J., Baskerville, C., Charbonneau, H., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2002). Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol. Biol. 3, 3. PMC / Journal
59. Shou, W., and Deshaies, R.J. (2002). Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet. 3, 4. PMC / Journal
58. Deshaies, R.J., Seol, J.H., McDonald, W.H., Cope, G., Lyapina, S.,Shevchenko, A., Shevchenko A., Verma, R., and Yates, J.R. (2002). Charting the protein complexome in yeast by mass spectrometry. Mol. Cell. Prot. 1, 3-10. Journal
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2001
57. Deshaies, R.J. and Ferrell, J.E. (2001). Multisite phosphorylation and the countdown to S phase. Cell 107, 819-822. Journal
56. Deshaies, R.J., and Eilers, M. (2001). Editorial Review: Cell Multiplication. Curr. Opin. Cell Biol. 13, 729-730. PDF
55. Wu, G., Lyapina, S., Das, I., Li, J., Guerney, M., Chui, I., Deshaies R.J., and Kitajewski, J. (2001). SEL-10, a negative regulator of notch signaling, targets notch for ubiquitin-mediated protein degradation. Mol. Cell Biol. 21, 7403-15. PMC / Journal
54. Verma, R., McDonald, W.H., Yates, J.R. III, and Deshaies, R.J. (2001). Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields fully active S phase cyclin-Cdk. Mol. Cell 8, 439-48. Journal
53. Shou, W., Sakamoto, K.M., Keener, J., Morimoto, K.W., Traverso, E., Azzam, R., Hoppe, G.J., Feldman, R.M., DeModena, J., Moazed, D., Charbonneau, H., Nomura, M., and Deshaies, R.J. (2001). Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Mol. Cell 8, 45-55. Journal
52. Zhou, C., Seibert, V., Geyer, R., Rhee, E., Lyapina, S., Cope, Greg., Deshaies R.J., Wolf, D.A. (2001). The fission yeast COP9/ signalosome is involved in cullin modification by ubiquitin-related Ned8p. BMC Biochem. 2, 7. PMC / Journal
51. Sakamoto, K.M., Kim, K.B., Kumagai, A., Mercurio, F., Crews, C.C. and Deshaies, R.J. (2001). Protacs: Chimeric molecules that target proteins to the Skp1-Cullin-F box complex for ubiquitination and degradation. Proc. Natl. Acad. Sci. USA 98, 8554-8559. PMC / Journal
50. Mah, A.S., Jang, J., and Deshaies, R.J. (2001). Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc. Natl. Acad. Sci. USA 13, 7325-30. PMC / Journal
49. Lyapina, S., Cope, G., Shevchenko, A., Serino, G., Tsuge, T., Zhou,C., Wolf, D.A., Wei, N., Shevchenko, A., and Deshaies, R.J. (2001). Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalsome. Science 18, 1382-5. Journal
48. Schwechheimer, C., Serino, G., Callis, J., Crosby, W.L., Lyapina, S., Deshaies, R.J., Gray W.M. Estelle M., Deng, X.W. (2001). Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response. Science 18, 1379-82. Journal
47. Chi, Y., Huddleston, M.J., Zhang, X., Young, R.A., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2001). Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase. Genes Dev. 9, 1078-92. PMC / Journal
46. Galan, J.-M., Wiederkehr, A., Seol, J.H., Deshaies, R.J., Riezman, H., and Peter, M. (2001). Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol. Cell Biol. 9, 3105-17. PMC / Journal
45. Seol, J.H., Shevchenko, A., Shevchenko, A., and Deshaies, R.J. (2001). Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat. Cell Biol. 4, 384-91. PDF
44. Traverso, E.E., Baskerville, C., Liu, Y., Shou, W., James, P., Deshaies, R.J., Charbonneau, H. (2001). Characterization of the net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. J. Biol. Chem. 276, 21924-31. Journal
43. Lippincott J., Shannon K.B., Shou W., Deshaies R.J., Li R. (2001). The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. J. Cell Sci. 7, 1379-86. Journal
42. Annan, R.S, Huddleston, M.J., Verma, R., Deshaies, R.J., and Carr, S.A. (2001). A multidimensional electrospray MS-based approach to phosphopeptide mapping. Anal. Chem. 73, 393-404. PDF
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2000
41. Blondel, M., Galan, J.M., Chi, Y., Lafourcade, C., Longaretti, C., Deshaies, R.J., and Peter, M. (2000). Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J. 19, 6085-6097. PMC / Journal
40. Verma, R., Chen, S., Feldman, R., Schieltz, D., Yates, J., and Deshaies, R.J. (2000). Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol. Biol. Cell 11, 3425-39. PMC / Journal
39. Reynard, G.J., Reynolds, W., Verma, R., and Deshaies, R.J. (2000). CKS1 is required for G1 cyclin/CDK activity in budding yeast. Mol. Cell Biol. 20, 5858-5864. PMC / Journal
38. Deshaies, R.J., and Meyerowitz, E.M. (2000). COP1 patrols the night beat. Nat. Cell Biol. 2, E102-E104. Journal
37. Verma, R., and Deshaies, R.J. (2000). A proteasome howdunit: the case of the missing signal. Cell 101, 341-344. Journal
1999
36. Deshaies, R.J. (1999). SCF and cullin/RING H2-based ubiquitin ligases. Annu. Rev. Cell Dev. Biol. 15, 435-467. Journal
35. Freed, E., Lacey, K.R., Huie, P., Lyapina, S.A., Deshaies, R.J., Stearns, T., and Jackson, P.K. (1999). Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13, 2242-2257. PMC / Journal
34. Seol, J.H., Feldman, R.M.R., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., Chi, Y., Galova, M., Claypool, J., Sandmeyer, S., Nasmyth, K., Shevchenko, A., and Deshaies, R.J. (1999). Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev. 13, 1614-1626. PMC / Journal
33. Straight, A.F., Shou, W., Dowd, G.J., Turck, C.W., Deshaies, R.J., Johnson, A.D., and Moazed, D. (1999). Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell 97, 245-256. Journal
32. Deshaies R.J., and Jacks T. (1999). Cell multiplication peering in and peering out: regulation of and by the cell cycle. Curr. Opin. Cell Biol. 11, 705-707. Journal
31. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W.S., Jang, J., Shevchenko, A., Charbonneau, H., and Deshaies, R.J. (1999). Exit from mitosis triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97, 233-244. Journal
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1998
30. Peters, J.-M., King, R.W., and Deshaies, R.J. (1998). Cell cycle control by ubiquitin-dependent proteolysis. In Ubiquitin and the Biology of the Cell (Ed. J.-M. Peters et al.) Plenum Press, NY. PDF
29. Lyapina, S.L., Correll, C.C., Kipreos, E.T., and Deshaies, R.J. (1998). Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein. Proc. Natl. Acad. Sci. USA 95, 7451-7456. PMC / Journal
1997
28. Henchoz, S., Chi, Y., Catarin, B., Herskowitz, I., Deshaies, R.J., and Peter, M. (1997). Phosphorylation and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev. 11, 3046-3060. PMC / Journal
27. Feldman, R.M.R., Correll, C.C., Kaplan, K.B., and Deshaies, R.J. (1997). A Complex of Cdc4, Skp1, and Cdc53/Cullin Catalyzes Ubiquitination of the Phosphorylated CDK Inhibitor Sic1. Cell 91, 221-230. Journal
26. Verma, R., Annan, R., Huddleston, M., Carr, S., Reynard, G., and Deshaies, R.J. (1997). Phosphorylation of Sic1p by G1 cyclin/Cdk is required for its degradation and entry into S phase. Science 278, 455-460. Journal
25. Verma, R., Feldman, R.M.R., and Deshaies, R. J. (1997). SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol. Biol. Cell 8, 1427-1437. PMC / Journal
24. Verma, R., Chi, Y., and Deshaies, R.J. (1997). Cell-free ubiquitination of cell cycle regulators in budding yeast extracts. Methods Enzymol. 283, 366-376. PDF
23. Deshaies, R.J. (1997). Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast. Curr. Opin. Genet. Dev. Biol. 7, 7-16. PDF
1996
22. King, R.W., Deshaies, R.J., Peters, J.M., and Kirschner, M.W. (1996). How proteolysis drives the cell cycle. Science 274, 1652-1659. PDF
1995
21. Deshaies, R.J. (1995). The self-destructive personality of a cell cycle in transition. Curr. Opin. Cell Biol. 7, 781-789. PDF
20. Deshaies, R.J. (1995). Make it or break it: the role of ubiquitin-dependent proteolysis in cellular regulation. Trends Cell Bio. 5, 428-434. PDF
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160. Verma R., Reichermeier K.M., Burroughs A.M., Oania R.S., Reitsma J.M., Aravind L., Deshaies R.J. (2018). Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes. Nature. 557, 446-51.
159. Reiterer V., Figueras-Puig C., Le Guerroue F., Confalonieri S., Vecchi M., Jalapothu D., Kanse S.M., Deshaies R.J., Di Fiore P.P., Behrends C., Farhan H. (2018). The pseudophosphatase STYX targets the F-box of FBXW7 and inhibits SCFFBXW7function. EMBO J. 15;37(6).
158. Liu X., Reitsma J.M., Mamrosh J.L., Zhang Y., Straube R., Deshaies R.J. (2018). Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression. Mol Cell. 69, 773-786.
2017
157. Skrott Z, Mistrik M, Andersen KK, Friis S, Majera D, Gursky J, Ozdian T, Bartkova J, Turi Z, Moudry P, Kraus M, Michalova M, Vaclavkova J, Dzubak P, Vrobel I, Pouckova P, Sedlacek J, Miklovicova A, Kutt A, Li J, Mattova J, Driessen C, Dou QP, Olsen J, Hajduch M, Cvek B, Deshaies RJ, Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature 552, 194-199.
156. Nakasone M.A., Lewis T.A., Walker O., Thakur A., Mansour W., Castañeda C.A., Goeckeler-Fried J.L., Parlati F., Chou T.F., Hayat O., Zhang D., Camara C.M., Bonn S.M., Nowicka U.K., Krueger S., Glickman M.H., Brodsky J.L., Deshaies R.J., Fushman D. (2017). Structural Basis for the Inhibitory Effects of Ubistatins in the Ubiquitin-Proteasome Pathway. Structure 25, 1839-1855.
155. Reitsma J.M., Liu X., Reichermeier K.M., Moradian A., Sweredoski M.J., Hess S., Deshaies R.J. (2017). Composition and Regulation of the Cellular Repertoire of SCF Ubiquitin Ligases. Cell. 171, 1326-1339.
154. Blythe, E.E., Olson, K.C., Chau, V. and Deshaies, R.J. (2017). Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP-NPLOC4-UFD1L is enhanced by a mutation that causes multisystem proteinpathy. Proc. Natl. Acad. Sci. U S A. E-published ahead of print May 16, 2017.
153. Lauinger, L., Li, J., Shostak, A., Cemel, I.A., Ha, N., Zhang, Y., Merkl, P.E., Obermeyer, S., Stankovic-Valentin, N., Schafmeier, T., Wever, W.J., Bowers, A.A., Carter, K.P., Palmer, A.E., Tschochner, H., Melchior, F., Deshaies, R.J., Brunner, M., and Diernfellner, A. (2017). Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat. Chem. Biol. E-published ahead of print May 1, 2017.
152. Nguyen, T.V., Li, J., Lu, C.J., Mamrosh, J.L., Lu, G., Cathers, B.E., and Deshaies, R.J. (2017). P97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc. Natl. Acad. Sci. U S A 14, 3565-3571.
151. Li, J., Yakushi, T., Parlati, F., Mackinnon, A.L., Perez, C., Ma, Y., Carter, K.P., Colayco, S., Magnuson, G., Brown, B., Nguyen, K., Vasile, S., Suyama, E., Smith, L.H., Sergienko, E., Pinkerton, A.B., Chung, T.D., Palmer, A.E., Pass, I., Hess, S., Cohen, S.M., and Deshaies RJ. (2017). Capzimin is a potent and specific inhibitor of proteasome isopeptidase Rpn11. Nat. Chem. Biol. 60, 1343-1361. Journal
150. Perez, C., Li, J., Parlati, F., Rouffet, M., Ma, Y., Mackinnon, A.L., Chou, T.F., Deshaies, R.J. and Cohen, S.M. (2017). Discovery of an Inhibitor of the Proteasome Subunit Rpn11. J. Med. Chem. 4, 1343-1361. Correction: J. Med. Chem. 7, 3217. Journal
2016
149. Reiterer, V, Figueras-Puig C., Le Guerroue F., Confalonieri, S., Vecchi, M., Jalapothu, D., Kanse, S.M., Deshaies, R.J., Di Fiore, P.P., Behrends, C. and Farhan, H. (2016). The pseudophosphatase STYX targets the F-box of FBXW7 and inhibits SCFFBXW7 function. EMBO J. 36, 260-273. Journal
148. Vangala, J.R, Sotzny, F., Kruger, E., Deshaies, R.J. and Radhakrishnan, S.K. (2016). Nrf1 can be processed and activated in a proteasome-independent manner. Curr. Biol. 26, R834-835. Journal
147. Kleiger, G. and Deshaies, R. (2016). Tag Team Ubiquitin Ligases. Cell 166, 1080-1081. Journal
146. Sung, M.K., Porras-Yakushi, T.R., Reitsma, J.M., Huber, F.M., Sweredoski, M.J., Hoelz, A., Hess, S. and Deshaies, R.J. (2016). A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. eLife 5, eLife.19105. PMC / Journal
145. Xue, L., Blythe, E.E., Freiberger, E.C., Mamrosh, J., Hebert, A.S., Reitsma, J.M., Hess, S., Coon, J.J. and Deshaies, R.J. VCP-adaptor interactions are exceptionally dynamic and subject to differential modulation by a VCP inhibitor. Mol. Cell. Proteomics 15 2970-2986. Journal
144. Sung, M.K., Reitsma, J.M., Sweredoski, M.J., Hess, S. and Deshaies, R.J. (2016). Ribosomal proteins produced in excess are degraded by the ubiquitin-proteasome system. Mol. Biol. 27, 2642-2652. PMC / Journal
143. Mosadeghi, R., Reichermeier, K.M., Winkler, M., Schreiber, A., Reitsma, J.M., Zhang, Y., Stengel, F., Cao, J., Kim, M., Sweredoski, M.J., Hess, S., Leitner, A., Aebersold, R., Peter, M., Deshaies, R.J. and Enchev, R.I. (2016). Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle. eLife 5, e12102. PMC / Journal
142. Nguyen, T.V., Lee, J.E., Sweredoski, M.J., Yang, S.J., Jeon, S.J., Harrison, J.S., Yim, J.H., Lee, S.G., Handa, H., Kuhlman, B., Jeong, J.S., Reitsma, J.M., Park, C.S., Hess, S. and Deshaies, R.J. (2016). Glutamine triggers acetylation-dependent degradation of glutamine synthetase via the thalidomide receptor cereblon. Mol. Cell 6, 809-820. Journal
141. Banerjee, S., Bartesaghi, A., Merk, A., Prashant, R., Bulfer, S. L., Yan, Y., Green, N., Mroczkowski, B., Neitz, R.J., Wipf, P., Falconieri, V., Deshaies, R.J., Milne, J.L.S., Huryn, D., Arkin, M. and Subramaniam, S. (2016). 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science 351, 871-875. Journal
2015
140. Alverez, C., Bulfer, S.L., Chakrasali, R., Chimenti, M.S., Deshaies R.J., Green, N., Kelly, M., LaPorte, M.G., Lewis, T.S., Liang, M., Moore, W.J., Neitz, R.J., Peshkov, V.A., Walters, M.A., Zhang, F., Arkin, M.R., Wipf, P. and Huryn, D.M. (2016). Allosteric indoleamide inhibitors of p97: Identification of a novel probe of the ubiquitin pathway. ACS Med. Chem. Lett. 2, 182-187. PMC / Journal
139. Deshaies, R.J. (2015). Protein degradation: Prime time for PROTACs. Nat. Chem. Biol. 11, 634-635. Journal
138. Pulvino, M., Chen, L., Oleksyn, D., Li, J., Compitello, G., Rossi, R., Spence, S., Balakrishnan, V., Jordan, C., Poligone, B., Casulo, C., Burack, R., Shapiro, J.L., Bernstein, S., Friedberg, J.W., Deshaies, R.J., Land, H., and Zhao, J. Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. (2015). Oncotarget 6, 14796-14813. PMC / Journal
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2014
137. Deshaies, R.J. (2014). Proteotoxic crisis, the ubiquitin-proteasone system, and cancer therapy. BMC Biol. 12:94. PMC / Journal
136. Fang, N.N., Chan, G.T., Zhu, M., Comyn, S.A., Persaud, A., Deshaies, R.J., Rotin, D., Gsponer, J., Mayor, T. (2014).
Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat. Cell Biol. 16, 1227-1237. PMC / Journal
135. Deshaies, RJ. Structural biology: Corralling a protein-degradation regulator. (2014). Nature 512, 145-146. Journal
134. Chan, N.C., den Besten, W., Sweredoski, M.J., Hess, S., Deshaies, R.J., and Chan, D.C. (2014). Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2. J. Biol. Chem. 289, 19789-19798. PMC / Journal
133. Chou, T.F., Bulfer, S.L., Weihl, C.C., Li, K., Lis, L.G., Walters, M.A., Schoenen, F.J., Lin, H.J., Deshaies, R.J., and Arkin, M.R. (2014). Specific inhibition of p97/VCP ATPase and kinetic analysis demonstrate interaction between D1 and D2 ATPase domains. J. Mol. Biol. 426, 2886-2899. PMC / Journal
132. Honarpour, N., Rose, C.M., Brumbaugh, J., Anderson, J., Graham, R.L., Sweredoski, M.J., Hess, S., Coon, J.J., and Deshaies, R.J. (2014). FBXL16 binds PP2A and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol. Cell. Proteomics 13, 780-791. PMC / Journal
131. Radhakrishnan, S.K., den Besten, W. and Deshaies, R.J. (2014). p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition. eLife 3, e01856. PMC / Journal
2013
130. Kolawa, N.J., Sweredoski, M.J., Graham, R.L.J., Oania, R., Hess, S., and Deshaies, R.J. (2013). Perturbations to the ubiquitin conjugate proteome in yeast Dubx mutants identify Ubx2 as a regulator of membrane lipid composition. Mol. Cell. Proteomics 12, 2791-2803. PMC / Journal
129. Kurimchak, A., Haines, D.S., Garriga, J., Wu, S., De Luca, F., Sweredoski, M.J., Deshaies, R.J., Hess, S., and Graña, A. (2013). Activation of p107 by FGF, which is essential for chondrocyte cell cycle exit, is mediated by the PP2A/B55a holoenzyme. Mol. Cell. Biol. 33, 3330-3342. PMC / Journal
128. Chou, T.F., Li, K., Nordin, B.E., Porubsky, P., Frankowski, K., Patricelli, M.P., Aubé, J., Schoenen, F.J., and Deshaies, R. (2013). Selective, reversible inhibitors of the AAA ATPase p97. Probe Reports from the NIH Molecular Libraries Program [Internet], National Center for Biotechnology Information, 2010-April 14, 2011 (updated May 1, 2013). Report
127. Pierce, N.W., Lee, J.E., Liu, X., Sweredoski, M.J., Graham, R.L., Larimore, E.A., Rome, M., Zheng, N., Clurman, B.E., Hess, S., Shan, S.O., and Deshaies, R.J. (2013). Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins. Cell 153, 206-215. PMC / Journal
126. Verma, R., Oania, R.S., Kolawa, N.J., and Deshaies, R.J. (2013). Cdc48/p97 promotes degradation of aberrant nascent polypeptides bound to the ribosome. eLife 2, e00308. PMC / Journal
125. Chou, T.F., Li, K., Frankowski, K.J., Schoenen, F.J., and Deshaies, R.J. (2013). Structure-activity relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase. Chem. Med. Chem. 8, 297-312. PMC / Journal
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2012
124. Oh, Y., Chang, K.J., Orlean, P., Wloka, C., Deshaies, R., and Bi, E. (2012). Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol. Biol. Cell. 23, 2445-2456. PMC / Journal
123. Emberley, E.D., Mosadeghi, R., and Deshaies, R.J. (2012). Deconjugation of Nedd8 from Cul1 is directly regulated by Skp1-Fbox and substrate, and CSN inhibits deneddylated SCF by a non-catalytic mechanism. J. Biol. Chem. 287, 29679-29689. PDF / Journal
122. Besten, W.D., Verma, R., Kleiger, G., Oania, R.S., and Deshaies, R.J. (2012). NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. Nat. Struct. Mol. Biol. 19, 511-516. PMC / Journal
121. Sohn, C.H., Agnew, H.D., Lee, J.E., Sweredoski, M.J., Graham, R.L., Smith, G.T., Hess, S., Czerwieniec, G., Loo, J.A., Heath, J.R., Deshaies, R.J., and Beauchamp, J.L. (2012). Designer reagents for mass spectrometry-based proteomics: clickable cross-linkers for elucidation of protein structures and interactions. Anal. Chem. 84, 2662-2669. PMC / Journal
120. Haines, D.S., Lee, J.E., Beauparlant, S.L., Kyle, D.B., den Besten, W., Sweredoski, M.J., Graham, R.L., Hess, S., and Deshaies, R.J. (2012). Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking. Mol. Cell. Proteomics 11, M111.016444. PMC / Journal
119. Sohn, C.H., Lee, J.E., Sweredoski, M.J., Graham, R.L., Smith, G.T., Hess, S., Czerwieniec, G., Loo, J.A., Deshaies, R.J., Beauchamp, J.L. (2012). Click chemistry facilitates formation of reporter ions and simplified synthesis of amine-reactive multiplexed isobaric tags for protein quantification. J. Am. Chem. Soc. 134, 2672-2680. PMC / Journal
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2011
118. Rabut, G., Le Dez, G., Verma, R., Makhneyvych, T., Knebel, A., Kurz, T., Boone, C., Deshaies, R.J. and Peter, M. (2011) The TFIIH subunit Tfb3 regulates cullin neddylation. Mol. Cell. 43, 488-495. PMC / Journal
117. Gomez, T.A., Kolawa, N., Gee, M., Sweredoski, M.J. and Deshaies, R.J. (2011). Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1. BMC Biol. 9, 33. PMC / Journal
116. Chou, T.F. and Deshaies, R.J. (2011). Development of p97 AAA ATPase inhibitors. Autophagy 7, 1091-1092. PMC / Journal
115. Chou, T.F. and Deshaies, R.J. (2011). Quantitative cell-based protein degradation assays to identify and classify drugs that target the ubiquitin-proteasome system. J. Biol. Chem. 286, 16546-16554. PMC / Journal
114. Lee, J.E., Sweredoski, M.J., Graham, R.L., Kolawa, N.J., Smith, G.T., Hess, S., and Deshaies, R.J. (2011). The steady-state repertoire of human SCF Ubiquitin ligase complexes does not require ongoing Nedd8 conjugation. Mol. Cell. Proteomics 10, 6460, 1-9. PMC / Journal
113. Saha, A., Lewis, S., Kleiger, G., Kuhlman, B., and Deshaies, R.J. (2011). Essential role for ubiquitin-ubiquitin-conjugating enzyme interaction in ubiquitin discharge from Cdc34 to substrate. Mol. Cell 42, 75-83. PMC / Journal
112. Chou, T.F., Brown, S.J., Minond, D., Nordin, B.E., Li, K., Jones, A.C., Chase, P., Porubsky, P.R., Stoltz, B.M., Schoenen, F.J., Patricelli, M.P., Hodder, P., Rosen, H., and Deshaies, R.J. (2011) Reversible inhibitor of p97, DBeQ, impairs both ubiquitin-dependent and autophagic protein clearance pathways. Proc. Natl. Acad. Sci. USA 108, 4834-4839. PMC / Journal
111. Verma, R., Oania, R., Fang, R., Smith, G.T., and Deshaies, R.J. (2011). Cdc48/p97 mediates UV-dependent turnover of RNA Pol II. Mol. Cell 41, 82-92. PMC / Journal
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2010
110. Deshaies, R.J., Emberley, E.D., and Saha, A. (2010). Control of cullin-ring ubiquitin ligase activity by Nedd8. Subcell. Biochem. 54, 41-56. Journal
109. Tyrell, A., Flick, K., Kleiger, G., Zhang, H., Deshaies, R.J. and Kaiser, P. (2010). Physiologically relevant and portable tandem ubiquitin-binding domain stabilizes polyubiquitylated proteins. Proc. Natl. Acad. Sci. USA 107, 19796-19801. PMC / Journal
108. Collins, G.A., Gomez, T.A., Deshaies, R.J., and Tansey, W.P. (2010). Combined chemical and genetic approach to inhibit proteolysis by the proteasome. Yeast 27, 965-974. PMC / Journal
107. Aghajan, M., Jonai, N., Flick, K., Fu, F., Luo, M., Cai, X., Ouni, I., Pierce, N., Tang, X., Lomenick, B., Damoiseaux, R., Hao, R., Del Moral, P.M., Verma, R., Li, Y., Li, C., Houk, K.N., Jung, M.E., Zheng, N., Huang, L., Deshaies, R.J., Kaiser, P., and Huang, J. (2010). Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase. Nat. Biotechnol. 28, 738-742. PMC / Journal
106. Radhakrishnan, S.K., Lee, C.S., Young, P., Beskow, A, Chan, J.Y., and Deshaies, R.J. (2010). Transcription factor Nrf1 mediates the proteasome recovery pathway after proteasome inhibition in mammalian cells. Mol. Cell 38, 17-28. PMC / Journal
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2009
105. Pierce, N., Kleiger, G., Shan, S., and Deshaies, R.J. (2009). Detection of sequential ubiquitination on a millisecond time-scale. Nature 462, 615-619. PMC / Journal
104. Kleiger, G., Saha, A., Lewis, S., Kuhlman, B., and Deshaies, R.J. (2009). Rapid E2-E3 assembly and disassembly enable processive ubiquitylation of cullin-RING ubiquitin ligase substrates. Cell 139, 957-968. PMC / Journal
103. Kleiger, G., Hao, B., Mohl, D.A., and Deshaies, R.J. (2009). The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCDC4. J. Biol. Chem. 284, 36012-36023. PMC / Journal
102. Deshaies, R.J. and Joazeiro, C.A.P. (2009). RING domain E3 ubiquitin ligases. Annu. Rev. Biochem. 78, 399-434. PDF
101. Deshaies, R.J. (2009). Drug discovery: Fresh target for cancer therapy (News & Views). Nature 458, 709-710. PDF
100. Oh, Y.M., Kwon, Y.E., Kim, J.M., Bae, S.J., Lee, B.K., Yoo, S.J., Chung, C.H., Deshaies R.J., Seol, J.H. (2009) Chfr is linked to tumour metastasis through the downregulation of HDAC1. Nat. Cell Biol. 11, 295-302. PDF
99. Machida, K., Tsukamoto, H., Mkrtchyan, H., Duan, L., Dynnyk, A., Liu, H.M., Asahina, K., Govindarajan, S., Ray, R., Ou, J.H., Seki, E., Deshaies, R., Miyake, K., and Lai, M.M. (2009). Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog. Proc. Nat. Acad. Sci. USA 106, 1548-1553. PMC / Journal
98. Mohl, D.A., Huddleston, M.J., Collingwood, T.S., Annan, R.S., and Deshaies, R.J. (2009). Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. J. Cell Biol. 184, 527-539. PMC / Journal
97. Collins, G.A., Lipford, J.R., Deshaies, R.J. and Tansey, W.P. (2009). Gal4 turnover and transcription activation (Brief Communication Arising). Nature 461, E7. PMC / Journal
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2008
96. Rodriguez-Gonzalez, A., Cyrus, K., Salcius, M., Kim, K., Crews, C.M., Deshaies, R.J., and Sakamoto, K.M. (2008). Targeting steroid hormone receptors for ubiquitination and degradation in breast and prostate cancer. Oncogene 27, 7201-7211. Journal
954. Saha, A. and Deshaies, R. J. (2008). Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. Mol. Cell 32, 21-31. PMC / Journal
94. Alexandru, G., Graumann, J., Smith, G.T., Kolawa, N.J., Fang, R., and Deshaies, R.J. (2008). UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1a turnover. Cell 134, 804-816. PMC / Journal
93. Goh, A.M., Walters, J.K., Elsasser, S., Verma, R., Deshaies, R.J., Finley, D., and Howley, P.M. (2008). Components of the ubiquitin-proteasome pathway compete for surfaces on Rad32 family proteins. BMC Biochem. 9, 4. PMC / Journal
92. Haririnia, A., Verma, R., Purohit, N., Twarog, M.Z., Deshaies, R.J., Bolon, D. and Fushman, D. (2008). Mutations in the hydrophobic core of ubiquitin differentially affect its recognition by receptor proteins. J. Mol. Biol. 375, 979-996. PMC / Journal
2007
91. Mayor, T., Graumann, J., Bryan, J., Maccoss, M.J., and Deshaies, R.J. (2007). Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway. Mol. Cell. Proteomics 6, 1885-1895. Journal
90. Ghaboosi, N. and Deshaies, R.J. (2007). A conditional yeast E1 mutant blocks the ubiquitin-proteasome pathway and reveals a role for ubiquitin conjugates in targeting Rad23 to the proteasome. Mol. Biol. Cell 18, 1953-63. PMC / Journal
2006
89. Petroski, M.D., Kleiger, G., and Deshaies, R.J. (2006). Evaluation of a diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex. Mol. Cell 24, 523-534. Journal
88. Sharon, M., Taverner, T., Ambroggio, X.I., Deshaies, R.J., and Robinson, C.V. (2006). Structural organization of the 19% proteasome lid: Insights from MS of intact complexes. PloS Biol. 4, 1314-1323 (e267). PMC / Journal
87. Cope, G.A. and Deshaies, R.J. (2006). Targeted silencing of JabI /Csn5 in human cells downregulates SCF activity through reduction of F-box protein levels. BMC Biochem. 7, 1. PMC / Journal
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2005
86. Petroski, M.D. and Deshaies, R.J. (2005). Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34. Cell 123, 1107-1120. Journal
85. Petroski, M.D. and Deshaies, R.J. (2005). In vitro reconstitution of SCF substrate ubiquitination with purified proteins. Method. Enzymol. 398, 143-158. PDF
84. Verma, R. and Deshaies, R.J. (2005). Assaying degradation and deubiquitination of a ubiquitinated substrate by purified 26S proteasomes. Method. Enzymol. 398, 391-399. PDF
83. Mayor, T. and Deshaies, R.J. (2005). Two-step affinity purification of multiubiquitylated proteins from Saccharomyces cerevisiae. Method. Enzymol. 399, 385-392. PDF
82. Mah, A.S., Elia, A.E.H., Devgan, G, Ptacek, J., Schutkowski, M., Snyder, M., Yaffe, M.B., and Deshaies, R.J. (2005). Substrate specificity analysis of protein kinase complex Dbf20Mob1 by peptide library and proteome array screening. BMC Biochem. 6, 22. PMC / Journal
81. Lipford, J.R., Smith, G.T., Chi, Y., and Deshaies, R.J. (2005). A putative stimulatory role for activator turnover in gene expression. Nature 438, 113-116. PDF
80. Mayor, T., Lipford, J.R., Graumann, J., Smith G.T., and Deshaies, R.J. (2005). Analysis of poly-ubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets. Mol. Cell. Proteomics 4, 741-751. Journal
79. Petroski, M.D. and Deshaies, R. J. (2005). Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell. Biol. 6, 9-20. PDF
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2004
78. Verma, R., Peters, N.R., Tochtrop, G.P., Sakamoto, K.M., D’Onofrio, M., Varadan, R., Fushman, D., Deshaies, R.J., and King, R.W. (2004). Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain. Science 306, 117-120. Journal
77. Azzam, R., Chen, S.L., Shou, W., Mah, A.S., Alexandru, G., Nasmyth, K., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2004). Phosphorylation by cyclin B-Cdk underlies release of mitotic exit. Science 305, 516-519. Journal
76. Verma, R., Oania, R., Graumann, J., and Deshaies, R.J. (2004). Multiubiquitin chain receptors define a layer of substrate selectivity in the ubiquitin-proteasome system. Cell 118, 99-110. Journal
75. Wertz, I.E., O'Rourke, K.M., Zhang, Z., Dornan, D., Arnott, D., Deshaies, R.J., and Dixit, V.M. (2004). Human De-etiolated-1 regulates cJun by assembling a CUL 4A ubiquitin ligase. Science 303, 1371-1374. Journal
74. Schneekloth, J.S. Jr., Fonseca, F.N., Koldobskiy, M., Mandal, A., Deshaies, R., Sakamoto, K. and Crews, C.M. (2004). Chemical genetic control of protein levels: selective in vivo targeted degradation. J. Am. Chem Soc. 126, 3748-3754. PDF
73. Graumann, J., Dunipace, L.A., Seol, J.H., McDonald, W.H., Yates, J.R. III, Wold, B.J., and Deshaies, R.J. (2004). Applicability of TAP-MudPIT to pathway proteomics in yeast. Mol. Cell. Proteomics 3, 266-237. Journal
72. Baumeister, W., Bachmair, A., Chau, V., Cohen, R., Coffino, P., DeMartino, G., Deshaies, R., et al. (2004). Varshavsky’s Contributions (in Letters). Science 19, 1290-1292. Journal
71. Ambroggio, X.I., Rees, D.C., and Deshaies, R.J. (2004). JAMM: A metalloprotease-like zinc site in the proteasome and signalosome. PLoS Biol. 2, 0113-0119. PMC / Journal
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2003
70. Sakamoto, K.M., Kim, K.B., Verma, R., Ransick, A., Stein, B., Crews, C.M., and Deshaies, R.J. (2003). Development of protacs to target cancer-promoting proteins for ubiquitination and degradation. Mol. Cell. Proteomics 2, 1350-1358. Journal
69. Lipford, J.R., and Deshaies, R.J. (2003). Diverse roles for ubiquitin-dependent proteolysis in transcriptional activation. Nat. Cell Biol. 5, 845-50. PDF
68. Cope, G.A., and Deshaies, R.J. (2003). COP9 signalosome: a multifunctional regulator of SCF and other cullin-based ubiquitin ligases. Cell 114, 663-71. Journal
67. Petroski, M.D, and Deshaies, R.J. (2003). Redundant degrons ensure the rapid destruction of sic1 at the g1 /s transition of the budding yeast cell cycle. Cell Cycle 2, 410-1. Journal
66. Petroski, M.D, and Deshaies, R.J. (2003). Context of multiubiquitin chain attachment influences the rate of Sic1 degradation. Mol. Cell 11, 1435-44. Journal
65. Park C.J., Song, S., Lee P.R., Shou W., Deshaies R.J. and Lee K.S (2003). Loss of CDC5 function in saccharomyces cerevisiae leads to defects in Swe1p regulation and Bfa1p/Bub2p-independent cytokinesis. Genetics 163, 21-33. PMC / Journal
2002
64. Cope, G.A., Suh, G.S., Aravind, L., Schwarz, S.E., Zipursky, S.L., Koonin, E.V. and Deshaies, R.J. (2002). Role of predicted metalloprotease motif of Jab1/Csn5 in cleavage of NEDD8 from CUL1. Science 298, 608-611. Journal
63. Verma, R., Aravind L., Oania, R., McDonald, W.H., Yates, J. R. III, Koonin, E.V. and Deshaies, R.J. (2002). Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome. Science 298, 611-615. Journal
62. Loughrey Chen S., Huddleston, M.J., Shou, W., Deshaies, R.J., Annan, R.S., and Carr, S.A. (2002). Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Mol. Cell. Prot. 1, 186-96. Journal
61. Shou, W., Verma, R., Annan, R.S., Huddleston, M.J., Chen, S.L., and Deshaies R.J. (2002). Mapping phosphorylation sites in proteins by mass spectrometry. Method. Enzymol. 351, 279-296. Preprint
60. Shou, W., Azzam, R., Chen, S.L., Huddleston, M.J., Baskerville, C., Charbonneau, H., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2002). Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol. Biol. 3, 3. PMC / Journal
59. Shou, W., and Deshaies, R.J. (2002). Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet. 3, 4. PMC / Journal
58. Deshaies, R.J., Seol, J.H., McDonald, W.H., Cope, G., Lyapina, S.,Shevchenko, A., Shevchenko A., Verma, R., and Yates, J.R. (2002). Charting the protein complexome in yeast by mass spectrometry. Mol. Cell. Prot. 1, 3-10. Journal
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2001
57. Deshaies, R.J. and Ferrell, J.E. (2001). Multisite phosphorylation and the countdown to S phase. Cell 107, 819-822. Journal
56. Deshaies, R.J., and Eilers, M. (2001). Editorial Review: Cell Multiplication. Curr. Opin. Cell Biol. 13, 729-730. PDF
55. Wu, G., Lyapina, S., Das, I., Li, J., Guerney, M., Chui, I., Deshaies R.J., and Kitajewski, J. (2001). SEL-10, a negative regulator of notch signaling, targets notch for ubiquitin-mediated protein degradation. Mol. Cell Biol. 21, 7403-15. PMC / Journal
54. Verma, R., McDonald, W.H., Yates, J.R. III, and Deshaies, R.J. (2001). Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields fully active S phase cyclin-Cdk. Mol. Cell 8, 439-48. Journal
53. Shou, W., Sakamoto, K.M., Keener, J., Morimoto, K.W., Traverso, E., Azzam, R., Hoppe, G.J., Feldman, R.M., DeModena, J., Moazed, D., Charbonneau, H., Nomura, M., and Deshaies, R.J. (2001). Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Mol. Cell 8, 45-55. Journal
52. Zhou, C., Seibert, V., Geyer, R., Rhee, E., Lyapina, S., Cope, Greg., Deshaies R.J., Wolf, D.A. (2001). The fission yeast COP9/ signalosome is involved in cullin modification by ubiquitin-related Ned8p. BMC Biochem. 2, 7. PMC / Journal
51. Sakamoto, K.M., Kim, K.B., Kumagai, A., Mercurio, F., Crews, C.C. and Deshaies, R.J. (2001). Protacs: Chimeric molecules that target proteins to the Skp1-Cullin-F box complex for ubiquitination and degradation. Proc. Natl. Acad. Sci. USA 98, 8554-8559. PMC / Journal
50. Mah, A.S., Jang, J., and Deshaies, R.J. (2001). Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc. Natl. Acad. Sci. USA 13, 7325-30. PMC / Journal
49. Lyapina, S., Cope, G., Shevchenko, A., Serino, G., Tsuge, T., Zhou,C., Wolf, D.A., Wei, N., Shevchenko, A., and Deshaies, R.J. (2001). Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalsome. Science 18, 1382-5. Journal
48. Schwechheimer, C., Serino, G., Callis, J., Crosby, W.L., Lyapina, S., Deshaies, R.J., Gray W.M. Estelle M., Deng, X.W. (2001). Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response. Science 18, 1379-82. Journal
47. Chi, Y., Huddleston, M.J., Zhang, X., Young, R.A., Annan, R.S., Carr, S.A., and Deshaies, R.J. (2001). Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase. Genes Dev. 9, 1078-92. PMC / Journal
46. Galan, J.-M., Wiederkehr, A., Seol, J.H., Deshaies, R.J., Riezman, H., and Peter, M. (2001). Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast. Mol. Cell Biol. 9, 3105-17. PMC / Journal
45. Seol, J.H., Shevchenko, A., Shevchenko, A., and Deshaies, R.J. (2001). Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat. Cell Biol. 4, 384-91. PDF
44. Traverso, E.E., Baskerville, C., Liu, Y., Shou, W., James, P., Deshaies, R.J., Charbonneau, H. (2001). Characterization of the net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. J. Biol. Chem. 276, 21924-31. Journal
43. Lippincott J., Shannon K.B., Shou W., Deshaies R.J., Li R. (2001). The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. J. Cell Sci. 7, 1379-86. Journal
42. Annan, R.S, Huddleston, M.J., Verma, R., Deshaies, R.J., and Carr, S.A. (2001). A multidimensional electrospray MS-based approach to phosphopeptide mapping. Anal. Chem. 73, 393-404. PDF
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2000
41. Blondel, M., Galan, J.M., Chi, Y., Lafourcade, C., Longaretti, C., Deshaies, R.J., and Peter, M. (2000). Nuclear-specific degradation of Far1 is controlled by the localization of the F-box protein Cdc4. EMBO J. 19, 6085-6097. PMC / Journal
40. Verma, R., Chen, S., Feldman, R., Schieltz, D., Yates, J., and Deshaies, R.J. (2000). Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol. Biol. Cell 11, 3425-39. PMC / Journal
39. Reynard, G.J., Reynolds, W., Verma, R., and Deshaies, R.J. (2000). CKS1 is required for G1 cyclin/CDK activity in budding yeast. Mol. Cell Biol. 20, 5858-5864. PMC / Journal
38. Deshaies, R.J., and Meyerowitz, E.M. (2000). COP1 patrols the night beat. Nat. Cell Biol. 2, E102-E104. Journal
37. Verma, R., and Deshaies, R.J. (2000). A proteasome howdunit: the case of the missing signal. Cell 101, 341-344. Journal
1999
36. Deshaies, R.J. (1999). SCF and cullin/RING H2-based ubiquitin ligases. Annu. Rev. Cell Dev. Biol. 15, 435-467. Journal
35. Freed, E., Lacey, K.R., Huie, P., Lyapina, S.A., Deshaies, R.J., Stearns, T., and Jackson, P.K. (1999). Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13, 2242-2257. PMC / Journal
34. Seol, J.H., Feldman, R.M.R., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., Chi, Y., Galova, M., Claypool, J., Sandmeyer, S., Nasmyth, K., Shevchenko, A., and Deshaies, R.J. (1999). Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev. 13, 1614-1626. PMC / Journal
33. Straight, A.F., Shou, W., Dowd, G.J., Turck, C.W., Deshaies, R.J., Johnson, A.D., and Moazed, D. (1999). Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell 97, 245-256. Journal
32. Deshaies R.J., and Jacks T. (1999). Cell multiplication peering in and peering out: regulation of and by the cell cycle. Curr. Opin. Cell Biol. 11, 705-707. Journal
31. Shou, W., Seol, J.H., Shevchenko, A., Baskerville, C., Moazed, D., Chen, Z.W.S., Jang, J., Shevchenko, A., Charbonneau, H., and Deshaies, R.J. (1999). Exit from mitosis triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97, 233-244. Journal
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1998
30. Peters, J.-M., King, R.W., and Deshaies, R.J. (1998). Cell cycle control by ubiquitin-dependent proteolysis. In Ubiquitin and the Biology of the Cell (Ed. J.-M. Peters et al.) Plenum Press, NY. PDF
29. Lyapina, S.L., Correll, C.C., Kipreos, E.T., and Deshaies, R.J. (1998). Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein. Proc. Natl. Acad. Sci. USA 95, 7451-7456. PMC / Journal
1997
28. Henchoz, S., Chi, Y., Catarin, B., Herskowitz, I., Deshaies, R.J., and Peter, M. (1997). Phosphorylation and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev. 11, 3046-3060. PMC / Journal
27. Feldman, R.M.R., Correll, C.C., Kaplan, K.B., and Deshaies, R.J. (1997). A Complex of Cdc4, Skp1, and Cdc53/Cullin Catalyzes Ubiquitination of the Phosphorylated CDK Inhibitor Sic1. Cell 91, 221-230. Journal
26. Verma, R., Annan, R., Huddleston, M., Carr, S., Reynard, G., and Deshaies, R.J. (1997). Phosphorylation of Sic1p by G1 cyclin/Cdk is required for its degradation and entry into S phase. Science 278, 455-460. Journal
25. Verma, R., Feldman, R.M.R., and Deshaies, R. J. (1997). SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol. Biol. Cell 8, 1427-1437. PMC / Journal
24. Verma, R., Chi, Y., and Deshaies, R.J. (1997). Cell-free ubiquitination of cell cycle regulators in budding yeast extracts. Methods Enzymol. 283, 366-376. PDF
23. Deshaies, R.J. (1997). Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast. Curr. Opin. Genet. Dev. Biol. 7, 7-16. PDF
1996
22. King, R.W., Deshaies, R.J., Peters, J.M., and Kirschner, M.W. (1996). How proteolysis drives the cell cycle. Science 274, 1652-1659. PDF
1995
21. Deshaies, R.J. (1995). The self-destructive personality of a cell cycle in transition. Curr. Opin. Cell Biol. 7, 781-789. PDF
20. Deshaies, R.J. (1995). Make it or break it: the role of ubiquitin-dependent proteolysis in cellular regulation. Trends Cell Bio. 5, 428-434. PDF
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Ray Deshaies: Graduate and Postdoctoral Publications
19. Banerjee, A., Deshaies, R.J., and Chau, V. (1995). Characterization of a dominant negative mutant of the cell cycle ubiquitin-conjugating enzyme Cdc34. J. Biol. Chem. 270, 26209-26215. Journal
18. Gerber, M.R., Farrell, A., Deshaies, R.J., Herskowitz, I. and Morgan, D.O. (1995). CDC37 is required for association of the protein kinase CDC28 with G1 and mitotic cyclins. Proc. Natl. Acad. Sci. USA 92, 4651-4655. PMC / Journal
17. Deshaies, R.J. and Kirschner, M.W. (1995). G1 cyclin-dependent activation of p34CDC28 in vitro. Proc. Natl. Acad. Sci. USA 92, 1182-1186. PMC / Journal
16. Deshaies, R.J., Chau, V., and Kirschner, M.W. (1995). Ubiquitination of the G1 cyclin Cln2p by a Cdc34p-dependent pathway. EMBO J. 14, 303-312. PMC
15. Li, J.J. and Deshaies, R. J. (1993). Exercising self-restraint: discouraging illicit acts of S and M in eukaryotes. Cell 74, 223-226. PDF
14. Booher, R.N., Deshaies, R.J. and Kirschner, M.W. (1993). Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J. 12, 3417-3426. PMC
13. Esnault, Y., Blondel, M.O., Deshaies, R.J., Schekman, R. and Kepes, F. (1993). The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum. EMBO J. 12, 4083-4093. PMC
12. Stirling, C.J., Rothblatt, J., Hosobuchi, M., Deshaies, R., and Schekman, R. (1992). Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell 3, 129-142. PMC / Journal
11. Deshaies, R.J., Sanders, S., Feldheim, D., and Schekman, R. (1991). Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature 349, 806-808. PDF
10. Deshaies, R.J. and Schekman, R. (1990). Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Mol. Cell. Biol. 10, 6024-6035. PMC / Journal
9. Rothblatt, J.A., Deshaies, R.J., Sanders, S.L., Daum, G. and Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell. Biol. 109, 2641-2652. PMC / Journal
8. Deshaies, R.J., and Schekman, R. (1989). SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum. J. Cell Biol. 109, 2653-2664. PMC / Journal
7. Deshaies, R.J., Kepes, F., and Bohni, P.C. (1989). Genetic dissection of the early stages of protein secretion in yeast. Trends Genet. 5, 87-93. PDF
6. Deshaies, R.J., Koch, B.D., and Schekman, R. (1988). The role of stress proteins in membrane biogenesis. Trends Biochem. Sci. 13, 384-388. PDF
5. Bohni, P.C., Deshaies, R.J., and Schekman, R. (1988). SEC11 is required for signal peptide processing and yeast cell growth. J. Cell Biol. 106, 1035-1042. PMC / Journal
4. Deshaies, R.J., Koch, B.D., Werner-Washburne, M., Craig, E.A., and Schekman, R. (1988). A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature 332, 800-805. PDF
3. Deshaies, R.J., and Schekman, R. (1987). A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J. Cell Biol. 105, 633-645. PMC / Journal
2. Deshaies, R.J., Fish, L.E., and Jagendorf, A.T. (1984). Permeability of chloroplast envelopes to Mg2+. Effects on protein synthesis. Plant Physiol. 74, 956-961. PMC / Journal
1. Fish, L.E., Deshaies, R., and Jagendorf, A.T. (1983). A Mg2+ requirement for rapid ATP-driven protein synthesis by intact pea chloroplasts. Plant Sci. Lett. 31, 139-146. PDF
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18. Gerber, M.R., Farrell, A., Deshaies, R.J., Herskowitz, I. and Morgan, D.O. (1995). CDC37 is required for association of the protein kinase CDC28 with G1 and mitotic cyclins. Proc. Natl. Acad. Sci. USA 92, 4651-4655. PMC / Journal
17. Deshaies, R.J. and Kirschner, M.W. (1995). G1 cyclin-dependent activation of p34CDC28 in vitro. Proc. Natl. Acad. Sci. USA 92, 1182-1186. PMC / Journal
16. Deshaies, R.J., Chau, V., and Kirschner, M.W. (1995). Ubiquitination of the G1 cyclin Cln2p by a Cdc34p-dependent pathway. EMBO J. 14, 303-312. PMC
15. Li, J.J. and Deshaies, R. J. (1993). Exercising self-restraint: discouraging illicit acts of S and M in eukaryotes. Cell 74, 223-226. PDF
14. Booher, R.N., Deshaies, R.J. and Kirschner, M.W. (1993). Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J. 12, 3417-3426. PMC
13. Esnault, Y., Blondel, M.O., Deshaies, R.J., Schekman, R. and Kepes, F. (1993). The yeast SSS1 gene is essential for secretory protein translocation and encodes a conserved protein of the endoplasmic reticulum. EMBO J. 12, 4083-4093. PMC
12. Stirling, C.J., Rothblatt, J., Hosobuchi, M., Deshaies, R., and Schekman, R. (1992). Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell 3, 129-142. PMC / Journal
11. Deshaies, R.J., Sanders, S., Feldheim, D., and Schekman, R. (1991). Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex. Nature 349, 806-808. PDF
10. Deshaies, R.J. and Schekman, R. (1990). Structural and functional dissection of Sec62p, a membrane-bound component of the yeast endoplasmic reticulum protein import machinery. Mol. Cell. Biol. 10, 6024-6035. PMC / Journal
9. Rothblatt, J.A., Deshaies, R.J., Sanders, S.L., Daum, G. and Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell. Biol. 109, 2641-2652. PMC / Journal
8. Deshaies, R.J., and Schekman, R. (1989). SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum. J. Cell Biol. 109, 2653-2664. PMC / Journal
7. Deshaies, R.J., Kepes, F., and Bohni, P.C. (1989). Genetic dissection of the early stages of protein secretion in yeast. Trends Genet. 5, 87-93. PDF
6. Deshaies, R.J., Koch, B.D., and Schekman, R. (1988). The role of stress proteins in membrane biogenesis. Trends Biochem. Sci. 13, 384-388. PDF
5. Bohni, P.C., Deshaies, R.J., and Schekman, R. (1988). SEC11 is required for signal peptide processing and yeast cell growth. J. Cell Biol. 106, 1035-1042. PMC / Journal
4. Deshaies, R.J., Koch, B.D., Werner-Washburne, M., Craig, E.A., and Schekman, R. (1988). A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature 332, 800-805. PDF
3. Deshaies, R.J., and Schekman, R. (1987). A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J. Cell Biol. 105, 633-645. PMC / Journal
2. Deshaies, R.J., Fish, L.E., and Jagendorf, A.T. (1984). Permeability of chloroplast envelopes to Mg2+. Effects on protein synthesis. Plant Physiol. 74, 956-961. PMC / Journal
1. Fish, L.E., Deshaies, R., and Jagendorf, A.T. (1983). A Mg2+ requirement for rapid ATP-driven protein synthesis by intact pea chloroplasts. Plant Sci. Lett. 31, 139-146. PDF
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