Protocols

Media​

• 5-FOA Agar
• Amino Acid Comparison
• Amino Acid Mix Powder
• Amino Acid Mix Solution
• Amino Acid Supplements
• Bacterial Stabs
• Cycloheximide (plates)
• Galactose 40%
• Glucose 40%
• LB Broth
• M63 Agar
• M63 Salts
• S Broth
• SD Agar
• SD Broth
• SOC/SOB
• Sorbitol 1M
• Sorbitol 2M
• TB Phosphate
• Terrific Broth
• Thiamine (Vit B1)
• YAPD Slants
• YEP Broth
• YEPD Agar
• YEPD Broth
• YEPD Freezing Solution
• zLA Schedule
• zPlate Assortment
• zPlouring Plates
• zTO DO

Reagents 

• Coomassie Blue Stain

• Destain Solution

• EDTA

• FBS Hanahan Tfn

• MgCl2

• MgSO4

• TA Buffer Stock

• TBE Buffer Stock

• TCA

• TO DO

Genetics

• Crossing Rho Mutant Strains
• LiAc yeast transformation
• TEST

Proteins

• Activation Extract Procedure
• Baculovirus End Point Dilution
• Concentrated Yeast Extract Prep
• Electrocompetant Bacteria
• Electroporation of Bacteria
• Fraction II prep
• GST Purification (Kellogg)
• Hanahan Competant Bacteria
• Hanahan Transformation
• Large Scale Cln2p translation
• SDS quickpreps
• Sporulation of Yeast
• TCA Quickpreps
• Transcription T7 Polymerase
• Translation in retic lysate

Protein Expression & Purifications

• Activation Extract Procedure

• Baculovirus End Point Dilution

• Concentrated Yeast Extract Prep

• Electrocompetent Bacteria

• Electroporation of Bacteria

• Fraction II prep

• GST Purification (Kellogg)

• Hanahan Competant Bacteria

• Hanahan Transformation

• Large Scale Cln2p translation

• SDS quickpreps

• Sporulation of Yeast

• TCA Quickpreps

• Transcription T7 Polymerase

• Translation in retic lysate

Western Blots

Tech

• Glass Bead Prep

• Glassware Sterilization

• Lab Surveillance

• Lab Tech Primer

• pH Meter Calibration

• Radiation Newsletter Notes

• Velvet Prep