THE DESHAIES LAB @ CALTECH
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Protocols

Media​
  • 5-FOA Agar
  • Amino Acid Comparison
  • Amino Acid Mix Powder
  • Amino Acid Mix Solution
  • Amino Acid Supplements
  • Bacterial Stabs
  • Cycloheximide (plates)
  • Galactose 40%
  • Glucose 40%
  • LB Broth
  • M63 Agar
  • M63 Salts
  • S Broth
  • SD Agar
  • SD Broth
  • SOC/SOB
  • Sorbitol 1M
  • Sorbitol 2M
  • TB Phosphate
  • Terrific Broth
  • Thiamine (Vit B1)
  • YAPD Slants
  • YEP Broth
  • YEPD Agar
  • YEPD Broth
  • YEPD Freezing Solution
  • zLA Schedule
  • zPlate Assortment
  • zPlouring Plates
  • zTO DO

Reagents 
  • Coomassie Blue Stain
  • Destain Solution
  • EDTA
  • FBS Hanahan Tfn
  • MgCl2
  • MgSO4
  • TA Buffer Stock
  • TBE Buffer Stock
  • TCA
  • TO DO
Genetics
  • Crossing Rho Mutant Strains
  • LiAc yeast transformation
  • TEST

Proteins
  • Activation Extract Procedure
  • Baculovirus End Point Dilution
  • Concentrated Yeast Extract Prep
  • Electrocompetant Bacteria
  • Electroporation of Bacteria
  • Fraction II prep
  • GST Purification (Kellogg)
  • Hanahan Competant Bacteria
  • Hanahan Transformation
  • Large Scale Cln2p translation
  • SDS quickpreps
  • Sporulation of Yeast
  • TCA Quickpreps
  • Transcription T7 Polymerase
  • Translation in retic lysate

Protein Expression & Purifications
  • Activation Extract Procedure
  • Baculovirus End Point Dilution
  • Concentrated Yeast Extract Prep
  • Electrocompetent Bacteria
  • Electroporation of Bacteria
  • Fraction II prep
  • GST Purification (Kellogg)
  • Hanahan Competant Bacteria
  • Hanahan Transformation
  • Large Scale Cln2p translation
  • SDS quickpreps
  • Sporulation of Yeast
  • TCA Quickpreps
  • Transcription T7 Polymerase
  • Translation in retic lysate

Western Blots

Tech
  • Glass Bead Prep
  • Glassware Sterilization
  • Lab Surveillance
  • Lab Tech Primer
  • pH Meter Calibration
  • Radiation Newsletter Notes
  • Velvet Prep
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